St. James City is a census-designated place on Pine Island in Lee County, Florida, United States. The population was 4,105 at the 2000 census. It is part of the Cape Coral-Fort Myers, Florida Metropolitan Statistical Area.St. James City is located at 26°32′11″N 82°5′29″W / 26.53639°N 82.09139°W / 26.53639; -82.09139.According to the United States Census Bureau, the CDP has a total area of 14.8 square miles, of which 14.6 square miles is land and 0.2 square miles is water.As of the census of 2000, there were 4,105 people, 2,138 households, and 1,417 families residing in the CDP. The population density was 281.1 people per square mile. There were 2,921 housing units at an average density of 200.0/sq mi. The racial makeup of the CDP was 98.95% White, 0.10% African American, 0.15% Native American, 0.27% Asian, 0.15% from other races, and 0.39% from two or more races. Hispanic or Latino of any race were 0.76% of the population.There were 2,138 households out of which 7.