Live Oak is a city in Suwannee County, Florida, United States. The city is the county seat of Suwannee County and is located east of Tallahassee. As of 2010, the population recorded by the U.S. Census Bureau was 6,850.U.S. Highway 90, U.S. Highway 129 and Interstate 10 are major highways running through Live Oak.It is served by the Suwannee County Airport as well as many private airparks scattered throughout the county.One other Florida county also has a community named Live Oak, which is located in Washington County.